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anti psrc  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti psrc
    Anti Psrc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 2356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psrc+tyr416/Phospho-Src+Family+(Tyr416)+Antibody/pmc12975496-86-23-24
    Average 96 stars, based on 2356 article reviews
    anti psrc - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Phospho-proteomics:

    Article Title: Protective Role of Endothelial Fibulin‐4 in Valvulo‐Arterial Integrity
    Article Snippet: .. Antibody Dilution Source Catalog number ACE 1:1000 Santa Cruz Biotechnology sc-20791 Thbs1 1:500 Neomarkers MS-421 Egr1 1:500 Cell Signaling #4154 pSmad 2 (Ser465/467) 1:500 Cell Signaling #3108 Smad2/3 1:1000 Cell Signaling #8685 pERK (Thr202/Tyr204) 1:500 Cell Signaling #4376 ERK1/2 1:1000 Cell Signaling #9102 pSrc (Tyr416) 1:500 Cell Signaling #6943 Src 1:1000 Cell Signaling #2109 GAPDH 1:1000 Cell Signaling #2118 ACE, angiotensin-converting enzyme; Thbs1, thrombospondin-1; Egr1, early growth response 1; p, phosphorylation; Smad, suppressor of mothers against decapentaplegic; ERK, extracellular signalregulated kinase; Src, proto‐oncogene tyrosine–protein kinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. .. Antibody Dilution Source Catalog number CD31 (PECAM) 1:100 or 1:200 BD Biosciences #550274 αSMA 1:200 or 1:300 Sigma-Aldrich A5228 CTGF 1:200 Santa Cruz Biotechnology sc-14939 Gdf15 1:100 Santa Cruz Biotechnology sc-515675 Hoechst 1:1000 Sigma-Aldrich B2261 CD31, cluster of differentiation 31; PECAM, platelet endothelial cell adhesion molecule 1; αSMA, αsmooth muscle actin; CTGF, connective tissue growth factor; Gdf15, growth differentiation factor-15.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Aspartate β-hydroxylase Regulates Expression of Ly6 Genes
    Article Snippet: After 24-h treatment with DMSO or the MO-I-1151 inhibitor, cells were lysed in lysis buffer (62.5 mM Tris pH 6.8, 20% glycerol, 4% (w/v) sodium dodecyl-sulfate (SDS) and protein concentration was quantified by a BCA protein assay (ThermoFisher Scientific). .. Equal amounts of proteins were resolved by 10% SDS polyacrylamide gel electrophoresis (SDS-PAGE), electroblotted to a polyvinylidene difluoride (PVDF) membrane, and blocked in 5% skimmed milk, followed by incubation with primary antibodies targeting mouse ASPH (cat. no. NBP1-69230, 1:1000; Novus Biologicals, Littleton, CO, USA; PB9478, 1:1000; Boster Biological Technology, Pleasanton, CA, USA); human ASPH (cat. no. NBP1-69229, 1:1000; Novus Biologicals); Notch1 (3608, 1:1000), activated-Notch1 (4147, 1:1000), HES1 (11988, 1:1000), Snail (3879, 1:500), Slug (9585, 1:500),Vimentin (5741, 1:1000), c-Myc (18583, 1:1000), Phospho-c-Myc-Thr58 (46650), SRC (2109, 1:1000), and pSRC-Tyr416 (6943, 1:1000) from Cell Signaling Technology (Danvers, MA, USA); Ly6a/Sca-1 (ab109211, 1:500; Abcam, Cambridge, UK); Ly6c (70362, 1:500; Elabscience, Houston, TX, USA); Ly6D (A17570, 1:1000; ABclonal Technology, Wuhan, China); Ly6K (sc-393560, 1:500; Santa Cruz Biotechnology; Dallas, TX, USA) and GAPDH (MA5-157380, 1:2000; ThermoFisher Scientific). ..

    Membrane:

    Article Title: Aspartate β-hydroxylase Regulates Expression of Ly6 Genes
    Article Snippet: After 24-h treatment with DMSO or the MO-I-1151 inhibitor, cells were lysed in lysis buffer (62.5 mM Tris pH 6.8, 20% glycerol, 4% (w/v) sodium dodecyl-sulfate (SDS) and protein concentration was quantified by a BCA protein assay (ThermoFisher Scientific). .. Equal amounts of proteins were resolved by 10% SDS polyacrylamide gel electrophoresis (SDS-PAGE), electroblotted to a polyvinylidene difluoride (PVDF) membrane, and blocked in 5% skimmed milk, followed by incubation with primary antibodies targeting mouse ASPH (cat. no. NBP1-69230, 1:1000; Novus Biologicals, Littleton, CO, USA; PB9478, 1:1000; Boster Biological Technology, Pleasanton, CA, USA); human ASPH (cat. no. NBP1-69229, 1:1000; Novus Biologicals); Notch1 (3608, 1:1000), activated-Notch1 (4147, 1:1000), HES1 (11988, 1:1000), Snail (3879, 1:500), Slug (9585, 1:500),Vimentin (5741, 1:1000), c-Myc (18583, 1:1000), Phospho-c-Myc-Thr58 (46650), SRC (2109, 1:1000), and pSRC-Tyr416 (6943, 1:1000) from Cell Signaling Technology (Danvers, MA, USA); Ly6a/Sca-1 (ab109211, 1:500; Abcam, Cambridge, UK); Ly6c (70362, 1:500; Elabscience, Houston, TX, USA); Ly6D (A17570, 1:1000; ABclonal Technology, Wuhan, China); Ly6K (sc-393560, 1:500; Santa Cruz Biotechnology; Dallas, TX, USA) and GAPDH (MA5-157380, 1:2000; ThermoFisher Scientific). ..

    Incubation:

    Article Title: Aspartate β-hydroxylase Regulates Expression of Ly6 Genes
    Article Snippet: After 24-h treatment with DMSO or the MO-I-1151 inhibitor, cells were lysed in lysis buffer (62.5 mM Tris pH 6.8, 20% glycerol, 4% (w/v) sodium dodecyl-sulfate (SDS) and protein concentration was quantified by a BCA protein assay (ThermoFisher Scientific). .. Equal amounts of proteins were resolved by 10% SDS polyacrylamide gel electrophoresis (SDS-PAGE), electroblotted to a polyvinylidene difluoride (PVDF) membrane, and blocked in 5% skimmed milk, followed by incubation with primary antibodies targeting mouse ASPH (cat. no. NBP1-69230, 1:1000; Novus Biologicals, Littleton, CO, USA; PB9478, 1:1000; Boster Biological Technology, Pleasanton, CA, USA); human ASPH (cat. no. NBP1-69229, 1:1000; Novus Biologicals); Notch1 (3608, 1:1000), activated-Notch1 (4147, 1:1000), HES1 (11988, 1:1000), Snail (3879, 1:500), Slug (9585, 1:500),Vimentin (5741, 1:1000), c-Myc (18583, 1:1000), Phospho-c-Myc-Thr58 (46650), SRC (2109, 1:1000), and pSRC-Tyr416 (6943, 1:1000) from Cell Signaling Technology (Danvers, MA, USA); Ly6a/Sca-1 (ab109211, 1:500; Abcam, Cambridge, UK); Ly6c (70362, 1:500; Elabscience, Houston, TX, USA); Ly6D (A17570, 1:1000; ABclonal Technology, Wuhan, China); Ly6K (sc-393560, 1:500; Santa Cruz Biotechnology; Dallas, TX, USA) and GAPDH (MA5-157380, 1:2000; ThermoFisher Scientific). ..

    Article Title: Heterogeneous Response of Tumor Cell Lines to Inhibition of Aspartate β-hydroxylase
    Article Snippet: .. The membranes were blocked in 5% skim milk, followed by an overnight incubation with primary antibodies against ASPH (NBP1-69229, 1:1000; Novus Biologicals, Littleton, CO, USA and PB9478, 1:1000; Boster Biological Technology, Pleasanton, CA, USA); Notch1 (3608, 1:1000), activated Notch1 (NICD; 4147, 1:1000), HES1 (11988, 1:1000), Snail (3879, 1:500), Slug (9585, 1:500), Vimentin (5741, 1:1000), c-myc (18583, 1:1000), phospho-c-myc-Thr58 (46650), SRC (2109, 1:1000), pSRC-Tyr416 (6943, 1:1000), pGSK3β (9322, 1:1000) from Cell Signaling Technology (Danvers, MA, USA); GSK3β (SAB5300175, 1:500) from Sigma‒Aldrich; cyclin D1 (sc-8396, 1:1000, Santa Cruz Biotechnology; Dallas, TX, USA); AKT (680302, 1:500; BioLegend; San Diego, CA, USA); pAKT- pS473 (AB1645553; 1:1000) BD Biosciences, Franklin Lakes, NJ, USA); and GAPDH (MA5-157380, 1:2000; ThermoFisher Scientific). ..

    Western Blot:

    Article Title: Deficiency of the Src homology phosphatase 2 in podocytes is associated with renoprotective effects in mice under hyperglycemia
    Article Snippet: .. Immunoblotting was performed using antibodies for pNF-κBp65 Ser536 (3033), NF-κBp65 (8242), pAKT Ser473 (4060), pERK1/2 Tyr202/Thr204 (4370), ERK1/2 (4695), pSrc Tyr416 (2101), pSrc Tyr527 (2105), PERK (3192), peIF2α Ser51 (9721), IRE1α (3294), GAPDH (3683) (all from Cell Signaling Technology), AKT (sc-81434), Shp2 (sc-280), pPERK Thr981 (sc-32577), eIF2α (sc-133132), TGFβRII (sc-17792), FYN (sc-73388), Actin (sc-47778), Tubulin (sc-8035), Nephrin (sc-19000) (all from Santa Cruz Biotechnology), pNephrin Tyr1176/Tyr1193 (Abcam; ab80299), and Synaptopodin and pIRE1α Ser724 (NB100–2323) from Novus Biologicals at 4°C overnight. ..



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